ELK8834-48T, Dog SDMA(Symmetric dimethylarginine) ELISA Kit, 48T

ELK8834-48T, Dog SDMA(Symmetric dimethylarginine) ELISA Kit, 48T

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ELK8834-96T, Dog SDMA(Symmetric dimethylarginine) ELISA Kit, 96T

2.963,10 RON

Dog SDMA(Symmetric dimethylarginine) ELISA Kit

SKU
ELK8834-96T

Alternative Names: Symmetric dimethylarginine

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Developmental science;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog SDMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Symmetric dimethylarginine

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Developmental science;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog SDMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.