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ELK8833-48T, ACTb(Actin Beta) ELISA Kit, 48T
2.142,00 RON
ACTb(Actin Beta) ELISA Kit
SKU
ELK8833-48T
Alternative Names: ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Developmental science;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed Species: General Assay Type: Competitive Inhibition Sensitivity: 0.057 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Developmental science;Bone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve. |
