Limba
Romanian
Romanian
English|
ELK8816-96T, Human SPX(Spexin) ELISA Kit, 96T
3.736,60 RON
Human SPX(Spexin) ELISA Kit
SKU
ELK8816-96T
Alternative Names: SPX; neuropeptide Q; NPQ
Species: Human
Assay Type: Sandwich
Sensitivity: 12.2 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Diabetes
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPX in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: SPX; neuropeptide Q; NPQ Species: Human Assay Type: Sandwich Sensitivity: 12.2 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Diabetes Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPX in the samples is then determined by comparing the OD of the samples to the standard curve. |
