ELK8811-48T, Rat ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit, 48T

Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.2 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADCYAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADCYAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADCYAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.