ELK8793-48T, Zebrafish Testo(Testosterone) ELISA Kit, 48T

Alternative Names: T; Testesteron; Testeronun

Species: Zebrafish

Assay Type: Competitive Inhibition

Sensitivity: 17.7 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: serum, plasma, other biological fluids

Assay length: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish Testo in the samples is then determined by comparing the OD of the samples to the standard curve.