ELK8780-48T, Duck MT(Melatonin) ELISA Kit, 48T

Alternative Names: N-Acetyl-5-Methoxytryptamine

Species: Duck

Assay Type: Competitive Inhibition

Sensitivity: 4.94 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Tumor immunity;Neuro science;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Duck MT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Duck MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Duck MT in the samples is then determined by comparing the OD of the samples to the standard curve.