ELK8768-48T, Cattle Estrogen ELISA Kit, 48T

Alternative Names: E

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 2h

Research Area: Reproductive science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.