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ELK8761-48T, Human E3 ubiquitin-protein ligase Mdm2,MDM2 ELISA Kit, 48T
2.142,00 RON
Human E3 ubiquitin-protein ligase Mdm2,MDM2 ELISA Kit
SKU
ELK8761-48T
Alternative Names: MDM2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.072 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, other biological fluids
Assay length: 3.5h
Research Area: Apoptosis
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Mdm2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Mdm2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Mdm2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Mdm2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: MDM2 Species: Human Assay Type: Sandwich Sensitivity: 0.072 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma, other biological fluids Assay length: 3.5h Research Area: Apoptosis Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Mdm2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Mdm2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Mdm2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Mdm2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
