ELK8730-48T, Dog hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit, 48T

Alternative Names: High Sensitivity Cardiac Troponin I

Species: Dog

Assay Type: Sandwich

Sensitivity: 4.94 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.