ELK8728-48T, Human PCSK6(Proprotein Convertase Subtilisin/Kexin Type 6) ELISA Kit, 48T

ELK8728-48T, Human PCSK6(Proprotein Convertase Subtilisin/Kexin Type 6) ELISA Kit, 48T

ELK8729-48T, Human SLAMF5(Signaling Lymphocytic Activation Molecule Family, Member 5) ELISA Kit, 48T

ELK8729-48T, Human SLAMF5(Signaling Lymphocytic Activation Molecule Family, Member 5) ELISA Kit, 48T

ELK8728-96T, Human PCSK6(Proprotein Convertase Subtilisin/Kexin Type 6) ELISA Kit, 96T

2.963,10 RON

Human PCSK6(Proprotein Convertase Subtilisin/Kexin Type 6) ELISA Kit

SKU
ELK8728-96T

Alternative Names: PACE4; SPC4; Paired Basic Amino Acid Cleaving System 4; Subtilisin-Like Proprotein Convertase 4; Subtilisin/Kexin-Like Protease PACE4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCSK6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCSK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCSK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCSK6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PACE4; SPC4; Paired Basic Amino Acid Cleaving System 4; Subtilisin-Like Proprotein Convertase 4; Subtilisin/Kexin-Like Protease PACE4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCSK6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCSK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCSK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCSK6 in the samples is then determined by comparing the OD of the samples to the standard curve.