ELK8716-48T, Rat T4(Thyroxine) ELISA Kit, 48T

Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 1.42 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.