Avian 5-HT(5-Hydroxytryptamine) ELISA Kit
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma
Species: Avian
Assay Type: Competitive Inhibition
Sensitivity: 3.97 ng/mL
Standard: 900 ng/mL
Detection range: 14.07-900 ng/mL
Sample type: serum and plasma
Assay length: 2h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.080,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma Species: Avian Assay Type: Competitive Inhibition Sensitivity: 3.97 ng/mL Standard: 900 ng/mL Detection range: 14.07-900 ng/mL Sample type: serum and plasma Assay length: 2h Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |