ELK8694-48T, Avian 5-HT(5-Hydroxytryptamine) ELISA Kit, 48T

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ELK8694-96T, Avian 5-HT(5-Hydroxytryptamine) ELISA Kit, 96T

2.475,20 RON

Avian 5-HT(5-Hydroxytryptamine) ELISA Kit

SKU
ELK8694-96T

Alternative Names: 5HT; Serotonin; N-Methyl-Gamma

Species: Avian

Assay Type: Competitive Inhibition

Sensitivity: 3.97 ng/mL

Standard: 900 ng/mL

Detection range: 14.07-900 ng/mL

Sample type: serum and plasma

Assay length: 2h

Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: 5HT; Serotonin; N-Methyl-Gamma

Species: Avian

Assay Type: Competitive Inhibition

Sensitivity: 3.97 ng/mL

Standard: 900 ng/mL

Detection range: 14.07-900 ng/mL

Sample type: serum and plasma

Assay length: 2h

Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.