ELK8687-96T, Rat Hyp(Hydroxyproline) ELISA Kit, 96T

Alternative Names: (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 26.3 ng/mL

Standard: 5000 ng/mL

Detection range: 78.13-5000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Nutrition metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.