ELK8678-48T, TMAO(Trimethylamine-N-oxide) ELISA Kit, 48T

ELK8678-48T, TMAO(Trimethylamine-N-oxide) ELISA Kit, 48T

ELK8679-48T, Horse cAMP(Cyclic Adenosine Monophosphate) ELISA Kit, 48T

ELK8679-48T, Horse cAMP(Cyclic Adenosine Monophosphate) ELISA Kit, 48T

ELK8678-96T, TMAO(Trimethylamine-N-oxide) ELISA Kit, 96T

2.963,10 RON

TMAO(Trimethylamine-N-oxide) ELISA Kit

SKU
ELK8678-96T

Alternative Names: TMAO

Species: General

Assay Type: Sandwich

Sensitivity: 4.93 ng/mL

Standard: 1000 ng/mL

Detection range: 15.63-1000 ng/mL

Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Immunology;Innate Immunity;Complement;Alternative Pathway, Immunology;Innate Immunity;Complement;Other

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TMAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TMAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TMAO in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: TMAO

Species: General

Assay Type: Sandwich

Sensitivity: 4.93 ng/mL

Standard: 1000 ng/mL

Detection range: 15.63-1000 ng/mL

Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Immunology;Innate Immunity;Complement;Alternative Pathway, Immunology;Innate Immunity;Complement;Other

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TMAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TMAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TMAO in the samples is then determined by comparing the OD of the samples to the standard curve.