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ELK8624-48T, Human Transcriptional regulator ATRX (ATRX) ELISA Kit, 48T
2.142,00 RON
Human Transcriptional regulator ATRX (ATRX) ELISA Kit
SKU
ELK8624-48T
Alternative Names: ATRX
Species: Human
Assay Type: Sandwich
Sensitivity: 0.082 ng/mL
Standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Glioma
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATRX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATRX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATRX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATRX in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ATRX Species: Human Assay Type: Sandwich Sensitivity: 0.082 ng/mL Standard: 20 ng/mL Detection range: 0.31-20 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Glioma Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATRX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATRX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATRX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATRX in the samples is then determined by comparing the OD of the samples to the standard curve. |
