ELK8601-48T, Biotin ELISA Kit, 48T

Alternative Names: Biotin

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.7 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 2h

Research Area: Enzyme & Kinase;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.