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ELK8583-96T, Chicken SAA(Serum Amyloid A) ELISA Kit, 96T
2.963,10 RON
Chicken SAA(Serum Amyloid A) ELISA Kit
SKU
ELK8583-96T
Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
Species: Chicken
Assay Type: Sandwich
Sensitivity: 0.1 µg/mL
Standard: 10 µg/mL
Detection range: 0.16-10 µg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken SAA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein Species: Chicken Assay Type: Sandwich Sensitivity: 0.1 µg/mL Standard: 10 µg/mL Detection range: 0.16-10 µg/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken SAA in the samples is then determined by comparing the OD of the samples to the standard curve. |
