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ELK8580-96T, Pig ADT(Androsterone) ELISA Kit, 96T
2.475,20 RON
Pig ADT(Androsterone) ELISA Kit
SKU
ELK8580-96T
Alternative Names: 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 144 pg/mL
Standard: 90000 pg/mL
Detection range: 1406.25-90000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Biochemical research
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ADT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ADT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ADT in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone Species: Pig Assay Type: Competitive Inhibition Sensitivity: 144 pg/mL Standard: 90000 pg/mL Detection range: 1406.25-90000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Biochemical research Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ADT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ADT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ADT in the samples is then determined by comparing the OD of the samples to the standard curve. |
