ELK8567-96T, Rat Acly(ATP-citRate synthase) ELISA Kit, 96T

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ELK8569-96T, Human Bap1(Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit, 96T

ELK8569-96T, Human Bap1(Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit, 96T

ELK8569-48T, Human Bap1(Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit, 48T

1.814,75 RON

Human Bap1(Ubiquitin carboxyl-terminal hydrolase BAP1) ELISA Kit

SKU
ELK8569-48T

Alternative Names: BAP1; BRCA1 associated protein 1; Cerebral protein 6; HUCEP 13; hucep 6; KIAA0272; UCHL2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Cancer

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bap1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bap1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bap1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bap1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: BAP1; BRCA1 associated protein 1; Cerebral protein 6; HUCEP 13; hucep 6; KIAA0272; UCHL2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Cancer

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bap1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bap1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bap1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bap1 in the samples is then determined by comparing the OD of the samples to the standard curve.