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ELK8552-48T, Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit, 48T
2.142,00 RON
Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit
SKU
ELK8552-48T
Alternative Names: CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F
Species: Rat
Assay Type: Sandwich
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Breast Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F Species: Rat Assay Type: Sandwich Sensitivity: 26.29 pg/mL Standard: 6000 pg/mL Detection range: 93.75-6000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Breast Cancer Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve. |
