ELK8536-96T, Human MICA(MHC class I chain-related protein A) ELISA Kit, 96T

Alternative Names: major histocompatibility complex class I chain-related gene A; MHC class I chain-related protein B; MHC class I polypeptide related sequence A; MHC class I polypeptide related sequence B; MIC-A

Species: Human

Assay Type: Sandwich

Sensitivity: 0.39 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Immunology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MICA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MICA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MICA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MICA in the samples is then determined by comparing the OD of the samples to the standard curve.