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ELK8532-96T, Human Muscarinic Acetylcholine Receptor (M-AChR) ELISA Kit, 96T
2.963,10 RON
Human Muscarinic Acetylcholine Receptor (M-AChR) ELISA Kit
SKU
ELK8532-96T
Alternative Names: M-AChR
Species: Human
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M-AChR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M-AChR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M-AChR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M-AChR in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: M-AChR Species: Human Assay Type: Sandwich Sensitivity: 0.135 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Infection immunity;Neuro science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M-AChR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M-AChR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M-AChR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M-AChR in the samples is then determined by comparing the OD of the samples to the standard curve. |
