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ELK8500-96T, Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) ELISA Kit, 96T
3.736,60 RON
Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) ELISA Kit
SKU
ELK8500-96T
Alternative Names: Pro-MMP-9
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.459 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Pro-MMP-9 Species: Mouse Assay Type: Sandwich Sensitivity: 0.459 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
