ELK8497-96T, Human HA(Hyaluronic Acid) ELISA Kit, 96T

Alternative Names: Hyaluronan; Hyaluronate

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.81 ng/mL

Standard: 400 ng/mL

Detection range: 6.25-400 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Tumor immunity;Hepatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HA in the samples is then determined by comparing the OD of the samples to the standard curve.