Limba
Romanian
Romanian
English|
ELK8474-96T, Pig MUC4(Mucin 4) ELISA Kit, 96T
2.963,10 RON
Pig MUC4(Mucin 4) ELISA Kit
SKU
ELK8474-96T
Alternative Names: ASGP; Mucin 4 Cell Surface Associated; Mucin 4,Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.131 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MUC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MUC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MUC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MUC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ASGP; Mucin 4 Cell Surface Associated; Mucin 4,Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin Species: Pig Assay Type: Sandwich Sensitivity: 0.131 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Tumor immunity;Infection immunity;Pulmonology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MUC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MUC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MUC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MUC4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
