Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Alternative Names: pMAPT /pTAU
Species: Human
Assay Type: Sandwich
Sensitivity: 12.37 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, other biological fluids
Assay length: 3.5h
Research Area: intracellular signal transduction and neuron's development and viability.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.490,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: pMAPT /pTAU Species: Human Assay Type: Sandwich Sensitivity: 12.37 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, other biological fluids Assay length: 3.5h Research Area: intracellular signal transduction and neuron's development and viability. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve. |