ELK8404-48T, Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit, 48T

Alternative Names: pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β

Species: Rat

Assay Type: Sandwich

Sensitivity: 16.4 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.