ELK8402-48T, Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit, 48T

Alternative Names: 8-isoprostane; 8-iso-PGF2α

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.2 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.