ELK8398-48T, HS(Heparan Sulfate) ELISA Kit, 48T

ELK8398-48T, HS(Heparan Sulfate) ELISA Kit, 48T

ELK8399-48T, Mouse GRIN1(Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit, 48T

ELK8399-48T, Mouse GRIN1(Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit, 48T

ELK8398-96T, HS(Heparan Sulfate) ELISA Kit, 96T

2.963,10 RON

HS(Heparan Sulfate) ELISA Kit

SKU
ELK8398-96T

Alternative Names: HS; Heparan Sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.19 ng/mL

Standard: 20 ng/mL

Detection range: 0.31-20 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HS in the samples is then determined by comparing the OD of the samples to the standard curve.

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Price 2.490,00 RON (preturile sunt fara TVA)
Description

Alternative Names: HS; Heparan Sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.19 ng/mL

Standard: 20 ng/mL

Detection range: 0.31-20 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HS in the samples is then determined by comparing the OD of the samples to the standard curve.