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ELK8398-96T, HS(Heparan Sulfate) ELISA Kit, 96T
2.963,10 RON
HS(Heparan Sulfate) ELISA Kit
SKU
ELK8398-96T
Alternative Names: HS; Heparan Sulfate
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.19 ng/mL
Standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HS in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HS; Heparan Sulfate Species: General Assay Type: Competitive Inhibition Sensitivity: 0.19 ng/mL Standard: 20 ng/mL Detection range: 0.31-20 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 2h Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HS in the samples is then determined by comparing the OD of the samples to the standard curve. |
