ELK8372-48T, Human 4-Hydroxynonenal(HNE) ELISA Kit, 48T

Alternative Names: 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 19.1 pg/mL

Standard: 2500 pg/mL

Detection range: 39.07-2500 pg/mL

Sample type: Serum, plasma, tissue homogenates

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.