Limba
Romanian
Romanian
English|
ELK8353-96T, Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit, 96T
2.963,10 RON
Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit
SKU
ELK8353-96T
Alternative Names: TG AB; TG-AB
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.64 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TG-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TG-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TG-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TG-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TG AB; TG-AB Species: Rat Assay Type: Sandwich Sensitivity: 0.64 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TG-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TG-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TG-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TG-Ab in the samples is then determined by comparing the OD of the samples to the standard curve. |
