Limba
Romanian
Romanian
English|
ELK8352-96T, Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit, 96T
2.963,10 RON
Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
SKU
ELK8352-96T
Alternative Names: TPO Ab; TPO-Ab
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 1.25 IU/mL
Standard: 80 IU/mL
Detection range: 1.25-80 IU/mL
Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TPO Ab; TPO-Ab Species: Rat Assay Type: Competitive Inhibition Sensitivity: 1.25 IU/mL Standard: 80 IU/mL Detection range: 1.25-80 IU/mL Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve. |
