ELK8351-48T, Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit, 48T

Alternative Names: 15-HETE; 15-Hydroxyeicosatetraenoic Acid)

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.1 ng/mL

Standard: 8 ng/mL

Detection range: 0.13-8 ng/mL

Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.