ELK8305-96T, Sheep RETN(Resistin) ELISA Kit, 96T

Alternative Names: ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

Species: Sheep

Assay Type: Sandwich

Sensitivity: 12.2 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep RETN in the samples is then determined by comparing the OD of the samples to the standard curve.