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ELK8302-96T, Sheep CRP(C-Reactive Protein) ELISA Kit, 96T
3.736,60 RON
Sheep CRP(C-Reactive Protein) ELISA Kit
SKU
ELK8302-96T
Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Species: Sheep
Assay Type: Sandwich
Sensitivity: 35 pg/mL
Standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sheep CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sheep CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sheep CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sheep CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1 Species: Sheep Assay Type: Sandwich Sensitivity: 35 pg/mL Standard: 5000 pg/mL Detection range: 78.13-5000 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay length: 3.5h Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sheep CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sheep CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sheep CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sheep CRP in the samples is then determined by comparing the OD of the samples to the standard curve. |
