Limba
Romanian
Romanian
English|
ELK8294-48T, Pig C3c(C3 Convertase) ELISA Kit, 48T
2.689,40 RON
Pig C3c(C3 Convertase) ELISA Kit
SKU
ELK8294-48T
Alternative Names: C4b2a
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.071 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig C3c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig C3c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig C3c in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.260,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: C4b2a Species: Pig Assay Type: Sandwich Sensitivity: 0.071 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay length: 3.5h Research Area: Signal transduction;Infection immunity;Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig C3c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig C3c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig C3c in the samples is then determined by comparing the OD of the samples to the standard curve. |
