Limba
Romanian
Romanian
English|
ELK8273-48T, S1P(Sphingosine-1-Phosphate) ELISA Kit, 48T
1.814,75 RON
S1P(Sphingosine-1-Phosphate) ELISA Kit
SKU
ELK8273-48T
Alternative Names: Sphingosine-1-Phosphate
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 4.62 ng/mL
Standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Ovarian dysfunction
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S1P protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Sphingosine-1-Phosphate Species: General Assay Type: Competitive Inhibition Sensitivity: 4.62 ng/mL Standard: 1000 ng/mL Detection range: 15.63-1000 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Ovarian dysfunction Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S1P protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve. |
