ELK8271-48T, CBG(Cinobufagin) ELISA Kit, 48T

Alternative Names: CBG

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 91.3 ng/mL

Standard: 20000 ng/mL

Detection range: 312.5-20000 ng/mL

Sample type: biological agents and other biological fluids

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CBG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CBG in the samples is then determined by comparing the OD of the samples to the standard curve.