ELK8254-48T, PCr(Phosphocreatine) ELISA Kit, 48T

Alternative Names: CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 49.3 ng/mL

Standard: 10000 ng/mL

Detection range: 156.25-10000 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PCr protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PCr. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCr in the samples is then determined by comparing the OD of the samples to the standard curve.