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ELK8250-96T, PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit, 96T
2.475,20 RON
PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit
SKU
ELK8250-96T
Alternative Names: PtdIns(3,4,5)P3
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 2.83 ng/mL
Standard: 500 ng/mL
Detection range: 7.82-500 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: PtdIns(3,4,5)P3 Species: General Assay Type: Competitive Inhibition Sensitivity: 2.83 ng/mL Standard: 500 ng/mL Detection range: 7.82-500 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Signal transduction; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
