NTM(Natamycin) ELISA Kit
Alternative Names: Pimaricin; Natacyn
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.45 ng/mL
Standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: biological agents
Assay length: 2h
Research Area: Infectious Diseases
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NTM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NTM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NTM in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.525,00 RON (preturile sunt fara TVA) |
---|---|
Description |
Alternative Names: Pimaricin; Natacyn Species: General Assay Type: Competitive Inhibition Sensitivity: 1.45 ng/mL Standard: 300 ng/mL Detection range: 4.69-300 ng/mL Sample type: biological agents Assay length: 2h Research Area: Infectious Diseases Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NTM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NTM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NTM in the samples is then determined by comparing the OD of the samples to the standard curve. |