ELK8173-48T, TCA(Taurocholic Acid) ELISA Kit, 48T

Alternative Names: Cholaic Acid; Cholyltaurine; Acidum Cholatauricum

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.41 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: Biological agents

Assay length: 2h

Research Area: Signal transduction;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TCA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TCA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TCA in the samples is then determined by comparing the OD of the samples to the standard curve.