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ELK8165-48T, Cattle HB(Hemoglobin) ELISA Kit, 48T
2.689,40 RON
Cattle HB(Hemoglobin) ELISA Kit
SKU
ELK8165-48T
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 5.57 µg/mL
Standard: 1000 µg/mL
Detection range: 15.63-1000 µg/mL
Sample type: Serum, plasma, erythrocyte lysates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.260,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2 Species: Cattle Assay Type: Competitive Inhibition Sensitivity: 5.57 µg/mL Standard: 1000 µg/mL Detection range: 15.63-1000 µg/mL Sample type: Serum, plasma, erythrocyte lysates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve. |
