ELK8151-96T, Mouse CLEC7A(C-Type Lectin Domain Family 7, Member A) ELISA Kit, 96T

Alternative Names: BGR; CLECSF1; BGR; CANDF4; CLECSF12; DECTIN1; Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: CD & Adhesion molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLEC7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLEC7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLEC7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLEC7A in the samples is then determined by comparing the OD of the samples to the standard curve.