ELK8146-96T, Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit, 96T

ELK8146-96T, Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit, 96T

ELK8147-96T, DAG(Diacylglycerol) ELISA Kit, 96T

ELK8147-96T, DAG(Diacylglycerol) ELISA Kit, 96T

ELK8147-48T, DAG(Diacylglycerol) ELISA Kit, 48T

1.814,75 RON

DAG(Diacylglycerol) ELISA Kit

SKU
ELK8147-48T

Alternative Names: DG; Diglyceride

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 141 pg/mL

Standard: 100000 pg/mL

Detection range: 1562.5-100000 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: DG; Diglyceride

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 141 pg/mL

Standard: 100000 pg/mL

Detection range: 1562.5-100000 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve.