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ELK8147-48T, DAG(Diacylglycerol) ELISA Kit, 48T
1.814,75 RON
DAG(Diacylglycerol) ELISA Kit
SKU
ELK8147-48T
Alternative Names: DG; Diglyceride
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 141 pg/mL
Standard: 100000 pg/mL
Detection range: 1562.5-100000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: DG; Diglyceride Species: General Assay Type: Competitive Inhibition Sensitivity: 141 pg/mL Standard: 100000 pg/mL Detection range: 1562.5-100000 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve. |
