ELK8145-96T, LPC(Lysophosphatidyl choline) ELISA Kit, 96T

ELK8145-96T, LPC(Lysophosphatidyl choline) ELISA Kit, 96T

ELK8146-96T, Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit, 96T

ELK8146-96T, Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit, 96T

ELK8146-48T, Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit, 48T

2.142,00 RON

Rat PRKAR2b(Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit

SKU
ELK8146-48T

Alternative Names: PRKAR2; RII-BETA

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRKAR2b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRKAR2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRKAR2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRKAR2b in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PRKAR2; RII-BETA

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRKAR2b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRKAR2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRKAR2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRKAR2b in the samples is then determined by comparing the OD of the samples to the standard curve.