ELK8144-96T, cGMP(Cyclic Guanosine Monophosphate) ELISA Kit, 96T

Alternative Names: c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 45.2 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;Metabolic pathway;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cGMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.