ELK8125-48T, VC(Vitamin C) ELISA Kit, 48T

Alternative Names: AA; L-Ascorbic Acid; Ascorbate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 192.1 ng/mL

Standard: 40000 ng/mL

Detection range: 625-40000 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Nutrition metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VC in the samples is then determined by comparing the OD of the samples to the standard curve.