ELK8124-48T, 5-HTP(5-Hydroxytryptophan) ELISA Kit, 48T

Alternative Names: Oxitriptan; Cincofarm; Levothym; Levotonine; Oxyfan; Telesol; Tript-OH; Triptum

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.77 ng/mL

Standard: 400 ng/mL

Detection range: 6.25-400 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve.