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ELK8115-48T, LPA(Lysophosphatidic Acid) ELISA Kit, 48T
1.814,75 RON
LPA(Lysophosphatidic Acid) ELISA Kit
SKU
ELK8115-48T
Alternative Names: LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 52.7 ng/mL
Standard: 10000 ng/mL
Detection range: 156.25-10000 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid Species: General Assay Type: Competitive Inhibition Sensitivity: 52.7 ng/mL Standard: 10000 ng/mL Detection range: 156.25-10000 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPA in the samples is then determined by comparing the OD of the samples to the standard curve. |
